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piercetm bca protein assay kit with an ionic detergent compatibility reagent  (Thermo Fisher)


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    Thermo Fisher piercetm bca protein assay kit with an ionic detergent compatibility reagent
    Piercetm Bca Protein Assay Kit With An Ionic Detergent Compatibility Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+reagent+piercetm+bca+protein+assay+kit/piercetm+bca+protein+assay+kit+with+an+ionic+detergent+compatibility+reagent/pm40603550-54-25-29
    Average 90 stars, based on 1 article reviews
    piercetm bca protein assay kit with an ionic detergent compatibility reagent - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Assessment of anionic siRNA lipoplexes prepared via modified ethanol injection for tumor cell delivery
    Article Snippet: AF555-fluorescence intensity [relative fluorescence unit (RFU)] in the supernatant were then measured using a fluorescence microplate reader (GloMax ® Discover system, Promega Corporation, Madison, WI, USA) at 525 nm of excitation and 580-640 nm of emission. .. Protein concentrations of the supernatants were determined with bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay Kit; Pierce; Thermo Fisher Scientific, Inc.). ..

    Article Title: Anticancer Activity of Novel Difluorinated Curcumin Analog and Its Inclusion Complex with 2-Hydroxypropyl-β-Cyclodextrin against Pancreatic Cancer
    Article Snippet: .. The protein estimation was performed using a BCA reagent (PierceTM BCA Protein Assay Kit) (ThermoScientific, Rockford, IL, USA), and 50 μg of protein for each group was used to load into the gels. .. Protein lysates were separated using polyacrylamide gel electrophoresis and transferred onto Immobilion membranes (PVDF, Millipore, Bedford, MA, USA).

    Article Title: Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method.
    Article Snippet: Aliquots of 10 μL of the supernatant were mixed with 50 μL of PicaGene MelioraStar-LT Luminescence Reagent (Toyo Ink Co., Ltd., Tokyo, Japan), and counts per second (cps) were measured using a chemoluminometer (ARVO X2; Perkin Elmer Co., Ltd., Waltham, MA, USA) as previously reported [28]. .. The protein concentration of each supernatant was determined using a bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay kit; Thermo Fisher Scientific, Inc.), and luciferase activity was calculated as cps/mg protein. ..

    Article Title: Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs.
    Article Snippet: Aliquots of 10 μl of the supernatants of cell lysates were mixed with 50 μl of PicaGene MelioraStar-lT luminescence reagent (Toyo ink Mfg. co., ltd.) and the luminescence was measured as counts per sec (cps) with a chemoluminometer (arVo X2; Perkinelmer, inc.). .. Protein concentrations of the supernatants were determined with bicinchoninic acid (Bca) reagent (PierceTM Bca Protein assay Kit; Pierce; Thermo Fisher Scientific, inc.), using bovine serum albumin (Thermo Fisher Scientific, Inc.) as a standard, and the luciferase activity (cps/μg protein) was calculated. luc activity (%) was calculated relative to that of untreated cells according to the formula below. luc activity (%)=(luc activity (cps/μg protein) in sirna transfected cells/luc activity (cps/μg protein) in untreated cells) x100. ..

    Article Title: Assessment of anionic siRNA lipoplexes prepared via modified ethanol injection for tumor cell delivery.
    Article Snippet: AF555‐fluorescence intensity [relative fluorescence unit (RFU)] in the supernatant were then measured using a fluorescence microplate reader (GloMax® Discover system, Promega Corporation, Madison, WI, USA) at 525 nm of excitation and 580‐640 nm of emission. .. Protein concentrations of the supernatants were determined with bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay Kit; Pierce; Thermo Fisher Scientific, Inc.). ..

    Protein Concentration:

    Article Title: Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method.
    Article Snippet: Aliquots of 10 μL of the supernatant were mixed with 50 μL of PicaGene MelioraStar-LT Luminescence Reagent (Toyo Ink Co., Ltd., Tokyo, Japan), and counts per second (cps) were measured using a chemoluminometer (ARVO X2; Perkin Elmer Co., Ltd., Waltham, MA, USA) as previously reported [28]. .. The protein concentration of each supernatant was determined using a bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay kit; Thermo Fisher Scientific, Inc.), and luciferase activity was calculated as cps/mg protein. ..

    Luciferase:

    Article Title: Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method.
    Article Snippet: Aliquots of 10 μL of the supernatant were mixed with 50 μL of PicaGene MelioraStar-LT Luminescence Reagent (Toyo Ink Co., Ltd., Tokyo, Japan), and counts per second (cps) were measured using a chemoluminometer (ARVO X2; Perkin Elmer Co., Ltd., Waltham, MA, USA) as previously reported [28]. .. The protein concentration of each supernatant was determined using a bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay kit; Thermo Fisher Scientific, Inc.), and luciferase activity was calculated as cps/mg protein. ..

    Article Title: Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs.
    Article Snippet: Aliquots of 10 μl of the supernatants of cell lysates were mixed with 50 μl of PicaGene MelioraStar-lT luminescence reagent (Toyo ink Mfg. co., ltd.) and the luminescence was measured as counts per sec (cps) with a chemoluminometer (arVo X2; Perkinelmer, inc.). .. Protein concentrations of the supernatants were determined with bicinchoninic acid (Bca) reagent (PierceTM Bca Protein assay Kit; Pierce; Thermo Fisher Scientific, inc.), using bovine serum albumin (Thermo Fisher Scientific, Inc.) as a standard, and the luciferase activity (cps/μg protein) was calculated. luc activity (%) was calculated relative to that of untreated cells according to the formula below. luc activity (%)=(luc activity (cps/μg protein) in sirna transfected cells/luc activity (cps/μg protein) in untreated cells) x100. ..

    Activity Assay:

    Article Title: Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method.
    Article Snippet: Aliquots of 10 μL of the supernatant were mixed with 50 μL of PicaGene MelioraStar-LT Luminescence Reagent (Toyo Ink Co., Ltd., Tokyo, Japan), and counts per second (cps) were measured using a chemoluminometer (ARVO X2; Perkin Elmer Co., Ltd., Waltham, MA, USA) as previously reported [28]. .. The protein concentration of each supernatant was determined using a bicinchoninic acid (BCA) reagent (PierceTM BCA Protein Assay kit; Thermo Fisher Scientific, Inc.), and luciferase activity was calculated as cps/mg protein. ..

    Article Title: Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs.
    Article Snippet: Aliquots of 10 μl of the supernatants of cell lysates were mixed with 50 μl of PicaGene MelioraStar-lT luminescence reagent (Toyo ink Mfg. co., ltd.) and the luminescence was measured as counts per sec (cps) with a chemoluminometer (arVo X2; Perkinelmer, inc.). .. Protein concentrations of the supernatants were determined with bicinchoninic acid (Bca) reagent (PierceTM Bca Protein assay Kit; Pierce; Thermo Fisher Scientific, inc.), using bovine serum albumin (Thermo Fisher Scientific, Inc.) as a standard, and the luciferase activity (cps/μg protein) was calculated. luc activity (%) was calculated relative to that of untreated cells according to the formula below. luc activity (%)=(luc activity (cps/μg protein) in sirna transfected cells/luc activity (cps/μg protein) in untreated cells) x100. ..

    Transfection:

    Article Title: Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs.
    Article Snippet: Aliquots of 10 μl of the supernatants of cell lysates were mixed with 50 μl of PicaGene MelioraStar-lT luminescence reagent (Toyo ink Mfg. co., ltd.) and the luminescence was measured as counts per sec (cps) with a chemoluminometer (arVo X2; Perkinelmer, inc.). .. Protein concentrations of the supernatants were determined with bicinchoninic acid (Bca) reagent (PierceTM Bca Protein assay Kit; Pierce; Thermo Fisher Scientific, inc.), using bovine serum albumin (Thermo Fisher Scientific, Inc.) as a standard, and the luciferase activity (cps/μg protein) was calculated. luc activity (%) was calculated relative to that of untreated cells according to the formula below. luc activity (%)=(luc activity (cps/μg protein) in sirna transfected cells/luc activity (cps/μg protein) in untreated cells) x100. ..



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